Islet biosample meta-data standards

This document describes recommended meta-data standards in PanKbase for reporting pancreatic islet biosamples from human donors

These recommendations are based on standards used by Human Islet Resource Network (HIRN) resources including the Integrated Islet Distribution Program (IIDP) and the Human Pancreas Analysis Program (HPAP), as well as the University of Alberta IsletCore. In addition, these standards consistent with those outlined in: Hart N, Powers A Diabetologia 2018

Biosamples are grouped into one of three ‘tiers’ based on the level of meta-data for the donor:

Required: the minimal required unit of meta-data for a biosample to be included in PanKbase

Comprehensive: a comprehensive set of meta-data expected for a biosample

Complete: a complete set of meta-data for a biosample, which may not be possible to achieve for all donors

Biosample meta-data records in PanKbase are annotated with the ‘tier’ that the biosample qualifies for

UUID - unique identifier describing the biosample (RRID)

Isolation center - center that islet sample was isolated from (fixed list)

Cold ischemia time - amount of time in cold ischemia (hr)

Organ source - source of the organ that the islets were isolated from (fixed list)

Islet preparation viability - viability of islets in the preparation (%)

Warm ischemia time - duration of warm ischemia/down time (hr)

Purity - islet preparation purity (%)

Hand picked - binary flag for whether the islets were hand picked or not (Y/N)

Pre-shipment culture time - amount of time that islets were culture before shipment (hr)

Islet function - description of how islet function was characterized (text field)

Digest time - amount of time for digestion (hr)

Percentage trapped - percentage of islets that were trapped (%)

Islet yield - islet equivalents obtained from isolation procedure (IEQs)

Pancreas weight - weight of the pancreas (g)