PKBDS9220VSGW

released

File Set Type

experimental data

Description

Dynamic islet perifusion assay for human donor RRID:SAMN15314807

Sample Summaries

SamplesReport view

1 item

Accession

Type

Sample Terms

Summary

PrimaryIslet

islet of Langerhans cell, Male, Homo sapiens

1 item

Accession

Sex

Taxa

Male

Homo sapiens

Were the Islets Shipped?

Yes

Islet Purification for Functional Assay

Hand-picked

Total Number of Islets for Functional Assay

110

Islet Size/Diameter (μm)

203.93

Islet Purity for Functional Assay (%)

100

Islet Endocrine Cell Composition (%)

beta:59%;alpha:36%;delta:4%

Perifusion Protocol

Base Media

5.6 mM

Stimuli or Secretagogues

16.7 mM glucose;16.7 mM glucose + 100 μM IBMX;1.7mM glucose + 1 μM Epinephrine;5.6 mM glucose + 20 mM KCl

Perifusion Flow Rate

Perifusion Fraction Volume

Fraction Collection Time (min)

Hormone Assays