EndoC-βH1 — PKBSM9156OROF

{
    "@context": "/terms/",
    "@id": "/human-beta-cell-line/PKBSM9156OROF/",
    "@type": [
        "HumanBetaCellLines",
        "Biosample",
        "Sample",
        "Item"
    ],
    "accession": "PKBSM9156OROF",
    "age": "unknown",
    "audit": {
        "INTERNAL_ACTION": [
            {
                "category": "mismatched status",
                "detail": "Current lab [kyle-gaulton](/labs/kyle-gaulton/) has deleted subobject award [U24DK1385121](/awards/U24DK1385121/).",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_item_status",
                "path": "/labs/kyle-gaulton/"
            }
        ],
        "WARNING": [
            {
                "category": "incorrect classifications format",
                "detail": "Human beta cell line [PKBSM9156OROF](/human-beta-cell-line/PKBSM9156OROF/) has `classifications` ['human beta cell line'] but should be [\"cell line\"]. Human beta cell lines are expected to have classifications as an array.",
                "level": 40,
                "level_name": "WARNING",
                "name": "audit_classifications_format_check",
                "path": "/human-beta-cell-line/PKBSM9156OROF/"
            }
        ]
    },
    "authentication": "not provided",
    "award": {
        "@id": "/awards/U24DK138512/",
        "component": "data coordination"
    },
    "classifications": [
        "human beta cell line"
    ],
    "coating_condition": "Matrigel-fibronectin",
    "creation_timestamp": "2025-10-01T04:30:13.055286+00:00",
    "description": "EndoC-bH1 cells with OAS1 expression vector from GSE244273",
    "donors": [
        {
            "@id": "/human-donors/PKBDO8293TLPA/",
            "accession": "PKBDO8293TLPA",
            "age": 0,
            "summary": "c4ca4606-3712-4fb6-b368-b8cec8bd7d08",
            "taxa": "Homo sapiens"
        }
    ],
    "file_sets": [],
    "growth_medium": "DMEM (glucose 1 g/L), BSA fraction V (2% wt/vol), 2-mercaptoethanol (50 μM), nicotinamide (10 mM), transferrin (5.5 μg/mL), sodium selenite (6.7 ng/mL), and PS (1%)",
    "institutional_certificates": [],
    "lab": {
        "@id": "/labs/kyle-gaulton/",
        "title": "Kyle Gaulton, UCSD"
    },
    "multiplexed_in": [],
    "nucleic_acid_delivery": "Lipofectamine 3000",
    "origin_of": [],
    "parts": [],
    "passage_number": "25-75",
    "pooled_in": [],
    "publication_identifiers": [
        "PMID:38409327"
    ],
    "release_timestamp": "2025-01-10T01:34:34.487993+00:00",
    "sample_name": "EndoC-bH1",
    "sample_terms": [
        {
            "@id": "/sample-terms/CVCL_909/",
            "term_name": "EndoC-βH1"
        }
    ],
    "sorted_fractions": [],
    "sources": [
        {
            "@id": "/sources/human-cell-design/",
            "title": "Human Cell Design"
        }
    ],
    "status": "released",
    "submitted_by": {
        "@id": "/users/7e95dcd6-9c35-4082-9c53-09d14c5752be/",
        "title": "Parul Kudtarkar"
    },
    "summary": "EndoC-βH1 cell line, Homo sapiens treated with 0 mg/kg OAS1 expression vector for 0 seconds, grown in DMEM (glucose 1 g/L), BSA fraction V (2% wt/vol), 2-mercaptoethanol (50 μM), nicotinamide (10 mM), transferrin (5.5 μg/mL), sodium selenite (6.7 ng/mL), and PS (1%)",
    "taxa": "Homo sapiens",
    "treatments": [
        {
            "@id": "/treatments/45ca0b7f-1ca6-48ad-ad98-08337d37fb03/",
            "purpose": "stimulation",
            "status": "released",
            "summary": "Treatment of 0 mg/kg OAS1 expression vector for 0 seconds",
            "treatment_type": "protein"
        }
    ],
    "uuid": "35f44cf9-8738-488a-a1b4-d123e4596c26",
    "virtual": false
}