EndoC-bH3 — PKBSM3459GAGF

{
    "@context": "/terms/",
    "@id": "/human-beta-cell-line/PKBSM3459GAGF/",
    "@type": [
        "HumanBetaCellLines",
        "Biosample",
        "Sample",
        "Item"
    ],
    "accession": "PKBSM3459GAGF",
    "age": "unknown",
    "aliases": [
        "pankbase-consortium:EndoC-bH3_treated_HNF1ACRISPR-Cas9_GSE277551"
    ],
    "audit": {
        "INTERNAL_ACTION": [
            {
                "category": "mismatched status",
                "detail": "Released human beta cell lines [PKBSM3459GAGF](/human-beta-cell-line/PKBSM3459GAGF/) has in progress subobject sample term [CVCL_IS72](/sample-terms/CVCL_IS72/).",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_item_status",
                "path": "/human-beta-cell-line/PKBSM3459GAGF/"
            }
        ],
        "WARNING": [
            {
                "category": "incorrect classifications format",
                "detail": "Human beta cell line [PKBSM3459GAGF](/human-beta-cell-line/PKBSM3459GAGF/) has `classifications` ['human beta cell line'] but should be [\"cell line\"]. Human beta cell lines are expected to have classifications as an array.",
                "level": 40,
                "level_name": "WARNING",
                "name": "audit_classifications_format_check",
                "path": "/human-beta-cell-line/PKBSM3459GAGF/"
            }
        ]
    },
    "award": {
        "@id": "/awards/U24DK138512-DK138515/",
        "component": "data coordination"
    },
    "classifications": [
        "human beta cell line"
    ],
    "coating_condition": "ECM, fibronectin",
    "creation_timestamp": "2025-10-21T21:46:51.530997+00:00",
    "description": "EndoC-bH3 cells treated with HNF1A sgRNA and CRISPR-Cas9 RNPs from GSE277551",
    "donors": [
        {
            "@id": "/human-donors/PKBDO8293TLPA/",
            "accession": "PKBDO8293TLPA",
            "age": 0,
            "summary": "PKBDO8293TLPA",
            "taxa": "Homo sapiens"
        }
    ],
    "excision_status": "unknown",
    "file_sets": [
        {
            "@id": "/analysis-sets/PKBDS3841GMJL/",
            "accession": "PKBDS3841GMJL",
            "aliases": [
                "pankbase-consortium:EndoC-bH3_treated_HNF1ACRISPR-Cas9_GSE277551_AnalysisSet"
            ],
            "lab": {
                "title": "Pankbase Consortium, PanKbase"
            },
            "status": "released",
            "summary": "intermediate analysis of RNA-seq data"
        },
        {
            "@id": "/measurement-sets/PKBDS5161ELPE/",
            "accession": "PKBDS5161ELPE",
            "aliases": [
                "pankbase-consortium:EndoC-bH3_treated_HNF1ACRISPR-Cas9_GSE277551_MeasurementSet"
            ],
            "assay_term": {
                "term_name": "bulk RNA-seq assay"
            },
            "lab": {
                "title": "Pankbase Consortium, PanKbase"
            },
            "status": "released",
            "summary": "bulk RNA-seq assay (RNA-seq)"
        }
    ],
    "growth_medium": "Advanced DMEM/F12 supplemented with 2% fatty acid-free Bovine Serum Albumin Fraction V, 6.7 ng/mL sodium selenite, 50 μM 2-mercaptoethanol, 5.5 μg/mL human transferrin, 10 mM nicotinamide, 1% GlutaMAX and 100 U/mL Penicillin-Streptomycin",
    "institutional_certificates": [],
    "lab": {
        "@id": "/labs/pankbase-consortium/",
        "title": "Pankbase Consortium, PanKbase"
    },
    "multiplexed_in": [],
    "origin_of": [],
    "parts": [],
    "pooled_in": [],
    "publication_identifiers": [
        "PMID:40774250"
    ],
    "release_timestamp": "2025-10-27T19:40:45.851802+00:00",
    "sample_name": "EndoC-bH3",
    "sample_terms": [
        {
            "@id": "/sample-terms/CVCL_IS72/",
            "term_name": "EndoC-bH3"
        }
    ],
    "sorted_fractions": [],
    "sources": [
        {
            "@id": "/sources/human-cell-design/",
            "title": "Human Cell Design"
        }
    ],
    "status": "released",
    "submitted_by": {
        "@id": "/users/5e3038c4-baa5-4589-b3d8-ad2fe634e826/",
        "title": "Ying Sun"
    },
    "summary": "EndoC-bH3 cell line, depleted of Cas9 RNP, HNF1A",
    "taxa": "Homo sapiens",
    "treatments": [
        {
            "@id": "/treatments/e01f3e09-969e-4d77-a94d-714c1d26d913/",
            "purpose": "stimulation",
            "status": "released",
            "summary": "Depletion of HNF1A",
            "treatment_type": "protein"
        },
        {
            "@id": "/treatments/b194d551-5b3a-4f9d-9ea9-c3a33e454829/",
            "purpose": "perturbation",
            "status": "released",
            "summary": "Depletion of Cas9 RNP",
            "treatment_type": "protein"
        }
    ],
    "uuid": "42595ce2-e537-4156-91ab-71fa693afa16",
    "virtual": false
}